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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
VECTOR SG Peroxidase (HRP) Substrate produces a blue-gray reaction product that can be used as a single label or as a second color for multiple antigen labeling. Vector SG substrates can be used for both manual and automated staining methods. Stained sections can be dehydrated, cleared, and permanently mounted.VECTOR SG Substrate Kit contains stock solutions in convenient dropper bottles. The kit provides the necessary reagents to prepare about 300 ml of working solution.
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Coelenterazine is a luminophore found in aquatic organisms and is the substrate for many luciferase enzymes, including Renilla reniformis, Gaussia and aequorins. It is commonly used for bioluminescent calcium detection and the monitoring of reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods as well as chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues.
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D-Luciferin potassium is the potassium salt of D-luciferin, the native substrate for firefly luciferase. It is provided as a water-soluble reagent for use in in vitro and in vivo bioluminescence assays and imaging, intended to deliver consistent luminescent signals with appropriate storage for stability.
Water-soluble potassium salt form suitable for aqueous assays.
Natural substrate for luciferase-based bioluminescent detection.
High purity (99.1% by LCMS) for reproducible assay results.
Human Factor X,100 U (0.8 mg),Buffer Composition = 20 mM Tris-HCl,0.1 M NaCl,1 mM Benzamidine,pH 7.4,Extinction Coefficient (1%) = 11.6,One Unit = 8 ug,Molecular Weight = 58,800 daltons,Prepared from fresh frozen human plasma
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Dabcyl–FRLKGGAPIKGV–Edans is an internally quenched fluorogenic substrate for the SARS-CoV or MERS-CoV family Papain-like protease (PLPro or PLP) that recognizes a LXGG consensus cleavage sites. Upon PLP-mediated cleavage between glycine and alanine, the resulting APIKGV-Edans fluorescence can be monitored at the excitation/emission wavelengths of 355/538 nm, respectively. In addition, PLP has deubiquitinating and deISG15ylation activities.
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A highly sensitive, stable substrate for HRP that reacts with H2O2 to produce the fluorescent compound resorufin; enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl samples
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Bacteroides Heparinase I, also called Heparin Lyase I, is active on heparin and the highly sulfated domains of heparan sulfate. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
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NEBExpress T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the beta 1-4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria). It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.
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An ideal alternative to other, more sensitive substrates where background is a problem. 4 CN (4-chloro-1-napthol) produces a precipitate at the reaction site in the presence of peroxidase-labeled conjugates. It is optimized for use in membrane assays and dot blots. 2-component format, simply mix and use.
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